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Plasmid 13544: pcDNA3.1 FLIPE-1m
  • FLIP-E A207W

  • ECFP-ybeJ-Venus

  • Glutamate/aspartate binding protein

  • gltI

  • 2400

  • E. coli

  • NP_415188

  • ECFP

  • N terminal on insert

  • Venus

  • C terminal on insert

  • A207W in binding protein

  • pcDNA3.1
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 5428

  • BamHI

  • No

  • HindIII

  • No

  • N/A List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • Venus is from Dr. Atsushi Miyawaki. Please ask Dr. Miyawaki for permission to use Venus in another vector.

  • View sequences (2)
  • Wolf Frommer

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

For more information, see
http://carnegiedpb.stanford.edu/research/fromme...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Okumoto et al (Proc Natl Acad Sci U S A. 2005 Jun 14. 102(24):8740-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13544" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only