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pcDNA3.1 FLIPE-1m
(Plasmid #13544)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    FLIP-E A207W
  • Alt name
    ECFP-ybeJ-Venus
  • Alt name
    Glutamate/aspartate binding protein
  • Alt name
    gltI
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    A207W in binding protein
  • GenBank ID
    NP_415188
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 FLIPE-1m was a gift from Wolf Frommer (Addgene plasmid # 13544)
  • For your References section:

    Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Okumoto S, Looger LL, Micheva KD, Reimer RJ, Smith SJ, Frommer WB. Proc Natl Acad Sci U S A. 2005 Jun 14. 102(24):8740-5. 10.1073/pnas.0503274102 PubMed 15939876