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pRSET FLIPE intramol-1m
(Plasmid #13551)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13551 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIP-E intramol A207W
  • Alt name
    ybeJ with ECFP inserted
  • Alt name
    Glutamate/aspartate binding protein
  • Alt name
    gltI
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    A207W, ECFP insert in binding protein
  • GenBank ID
    NP_415188
  • Entrez Gene
    gltI (a.k.a. b0655, ECK0648, JW5092, ybeJ, yzzK)
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FLIPE intramol-1m was a gift from Wolf Frommer (Addgene plasmid # 13551 ; http://n2t.net/addgene:13551 ; RRID:Addgene_13551)
  • For your References section:

    Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB. Protein Sci. 2005 Sep . 14(9):2304-14. 10.1110/ps.051508105 PubMed 16131659