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Plasmid 13560: pRSET FLIPglu-3.2mDelta13
  • FLIPglu F16A S112A Delta13

  • ECFP-mglB-EYFP

  • Periplasmic glucose binding

  • 2400

  • E. coli

  • ECFP

  • N terminal on insert

  • EYFP

  • C terminal on insert

  • F16A, S112A in binding protein

  • pRSET-B
    (Search Vector Database)

  • Bacterial Expression

  • 2897

  • BamHI

  • No

  • HindIII

  • No

  • N/A List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Wolf Frommer

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

For more information, see
http://carnegiedpb.stanford.edu/research/fromme...

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Deuschle et al (Protein Sci. 2005 Sep . 14(9):2304-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13560" in your Materials and Methods section.