|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13560||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2897
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFLIPglu F16A S112A Delta13
Alt namePeriplasmic glucose binding
Insert Size (bp)2400
MutationF16A, S112A in binding protein
/ Fusion Proteins
- ECFP (N terminal on insert)
- EYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
For more information, see
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSET FLIPglu-3.2mDelta13 was a gift from Wolf Frommer (Addgene plasmid # 13560)
For your References section:Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB. Protein Sci. 2005 Sep . 14(9):2304-14. 10.1110/ps.051508105 PubMed 16131659
Generated by Addgene from full sequence supplied by depositor.