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pRSET FLIPglu-3.2mDelta13
(Plasmid #13560)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    FLIPglu F16A S112A Delta13
  • Alt name
    ECFP-mglB-EYFP
  • Alt name
    Periplasmic glucose binding
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    F16A, S112A in binding protein
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FLIPglu-3.2mDelta13 was a gift from Wolf Frommer (Addgene plasmid # 13560)
  • For your References section:

    Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB. Protein Sci. 2005 Sep . 14(9):2304-14. 10.1110/ps.051508105 PubMed 16131659