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pAAV-Sox2-CreERT2
(Plasmid #135618)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135618 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene 61591
  • Backbone size w/o insert (bp) 3883
  • Total vector size (bp) 6947
  • Modifications to backbone
    Contains a 981 bp stuffer sequence 3' of the insert
  • Vector type
    Mouse Targeting, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    P2A-CreERT2 flanked by Sox2 homology arms
  • Alt name
    Sox2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3064
  • GenBank ID
    20674
  • Entrez Gene
    Sox2 (a.k.a. Sox, Sox-2, lc, lcc, ys, ysb)
  • Tag / Fusion Protein
    • P2A-CreERT2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Sox2-CreERT2 was a gift from Lin He (Addgene plasmid # 135618 ; http://n2t.net/addgene:135618 ; RRID:Addgene_135618)
  • For your References section:

    CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection. Chen S, Sun S, Moonen D, Lee C, Lee AY, Schaffer DV, He L. Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103. 10.1016/j.celrep.2019.05.103 PubMed 31242412