|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13566||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2897
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFLIPglu F16A P32S-ECFP-D33R
Alt namemglB with ECFP inserted
Alt namePeriplasmic glucose binding
Insert Size (bp)2400
MutationF16A, ECFP insert in binding protein
/ Fusion Proteins
- 6xHis (N terminal on insert)
- Citrine (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byCitrine is from Roger Tsien, UCSD.
Terms and Licenses
- Not Available to Industry
For more information, see
Citrine is modified version of EYFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSET FLII32Pglu-2.2m was a gift from Wolf Frommer (Addgene plasmid # 13566 ; http://n2t.net/addgene:13566 ; RRID:Addgene_13566)
For your References section:Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB. Protein Sci. 2005 Sep . 14(9):2304-14. 10.1110/ps.051508105 PubMed 16131659