Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pRSET FLII32Pglu-2.2m
(Plasmid #13566)


Item Catalog # Description Quantity Price (USD)
Plasmid 13566 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    FLIPglu F16A P32S-ECFP-D33R
  • Alt name
    mglB with ECFP inserted
  • Alt name
    Periplasmic glucose binding
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
    F16A, ECFP insert in binding protein
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • Citrine (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information, see

Citrine is modified version of EYFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FLII32Pglu-2.2m was a gift from Wolf Frommer (Addgene plasmid # 13566 ; ; RRID:Addgene_13566)
  • For your References section:

    Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB. Protein Sci. 2005 Sep . 14(9):2304-14. 10.1110/ps.051508105 PubMed 16131659