V406 4HA-CKIe(K38R)
(Plasmid #13725)

• Depositing Lab: David Virshup
• Publication: Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-4.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: casein kinase 1 epsilon
• Alt name: CKIe
• Alt name: CK1e
• Alt name: casein kinase i epsilon
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Mutation: kinase-inactive due to K38R mutation
• GenBank ID: L37043
• Entrez Gene: CSNK1E (a.k.a. CKIe, CKIepsilon, HCKIE)
• Tag / Fusion Protein:
  • 4 HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SfiI (not destroyed)
• 5′ sequencing primer: pCEP forward
• 3′ sequencing primer: EBV Reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.