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ACS1
(Plasmid #13746)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pQE80
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Acetyl CoA Synthetase 1
  • Alt name
    Acss2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2103
  • GenBank ID
    NM_019811
  • Entrez Gene
    Acss2 (a.k.a. ACAS, Acs1, Acas1, Acas2, AceCS1, 1110017C11Rik)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pQE80 sequencing vector
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ACS1 was a gift from John Denu (Addgene plasmid # 13746)
  • For your References section:

    Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Hallows WC, Lee S, Denu JM. Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5. 10.1073/pnas.0604392103 PubMed 16790548