|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13780||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3100
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNrl promoter-Cre
SpeciesM. musculus (mouse)
Insert Size (bp)4308
Entrez GeneNrl (a.k.a. D14H14S46E)
/ Fusion Protein
- Cre (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template.
Terms and Licenses
Mouse Nrl promoter was isolated by genomic PCR using B6 mouse genomic DNA as a template.
The Nrl promoter-Cre is specific for rod photorecetor cells in the retina.
Cre has a Myc-tag at its C-terminus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNrl-Cre was a gift from Connie Cepko (Addgene plasmid # 13780 ; http://n2t.net/addgene:13780 ; RRID:Addgene_13780)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010