RCAS (A) Flu cPitx2 (CT#704)
(Plasmid #13928)

• Depositing Lab: Cliff Tabin
• Publication: Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Backbone

• Vector backbone: RCASBP(A)
• Backbone size w/o insert (bp): 11600
• Vector type: Retroviral ; avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pitx2
• Alt name: Ptx2
• Species: G. gallus (chicken)
• Entrez Gene: PITX2
• Tag / Fusion Protein:
  • Flu (3xHA) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: RCAS-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The entire open reading frame of cPitx2 was generated by high fidelity PCR and cloned into a version of pSlax13 containing a triple HA (Flu) tag (Laufer et al., 1997). The sequence of the PCR-generated insert was confirmed by sequencing. A ClaI fragment containing the cPitx2-Flu sequence was cloned into the RCAS retroviral vector.