Addgene - RCAS(A) dRhoA V14 (CT#846) Plasmid Data

Plasmid 13949: RCAS(A) dRhoA V14 (CT#846)

Gene/insert name:
RhoA
Alt name:
RhoA-V14
Alt name:
Activated RhoA
Insert size:
600
Species:
D. melanogaster (fly)
Entrez Gene:
Rho1 (Dmel_CG8416, 8416, AAF01186, CG8416, D-Rho1, DRho, DRho1, DRhoA, Dm Rho1, DmRHO-A, Dmel\CG8416, Drho1, DrhoA, E3.10/J3.8, RHO, RHO1, Rho, Rho A, Rho GTPase, Rho kinase, RhoA, RhoN19, dRho1, dRhoA, dnRho, l(2)52Fa, l(2)k02107b, n(2)k07236, rho, rho1, rhoA)
Mutation:
G14V mutation
Vector backbone:
RCASBP(A)
(Search Vector Database)
Vector type:
Retroviral ; avian expression
Backbone size w/o insert (bp):
11600
Cloning site 5':
ClaI
Site destroyed during cloning:
No
Cloning site 3':
ClaI
Site destroyed during cloning:
No
5' sequencing primer:
RCAS-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Cliff Tabin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Wnt-14 plays a pivotal role in inducing synovial joint formation in the developing appendicular skeleton. Hartmann et al (Cell. 2001 Feb 9. 104(3):341-51. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13949" in your Materials and Methods section.