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pSLAX13 Flag (CT#231)
(Plasmid #14026)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14026 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • Flag

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.

pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.

For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLAX13 Flag (CT#231) was a gift from Cliff Tabin (Addgene plasmid # 14026 ; http://n2t.net/addgene:14026 ; RRID:Addgene_14026)
  • For your References section:

    Targeted gene misexpression in chick limb buds using avian replication-competent retroviruses. Logan M, Tabin C. Methods. 1998 Apr . 14(4):407-20. 10.1006/meth.1998.0595 PubMed 9608511