RCASBP (E) (CC#36)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15163||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneRCASBP (E)
- Backbone size (bp) 12000
Vector typeRetroviral ; Avian expression
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byRCASBP(A) provided by S. Hughes.
Terms and Licenses
The name RCAS stands for Replication-Competent ASLV long terminal repeat (LTR) with a Splice acceptor. The RCAS vectors retain the src splice site and express an inserted gene via a spliced message. These vectors will replicate in appropriately chosen avian cells (usually chicken or quail, although others can be used). In some cases, RCAS vectors will infect, but will not replicate, in mammalian cells. Please also see the paper Hughes SH et al., J Virol 1987 Oct; 61(10):3004-3012.
We constructed a fourth vector of the RCAS series of the E subgroup. Two hypervariable regions within the extracellular domain of the env gene are of particular importance in determining the host range of different viral subgroups. Starting with RCASBP(A), a new vector of the E subgroup, called RCASBP(E), was constructed by swapping a portion of the A env with E env. The E env sequences were obtained from pRAV-0 by KpnI-SalI digestion and isolation of the 1.1-kb env fragment. This fragment was ligated to RCASBP(A) in place of the homologous KpnI-SalI fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCASBP (E) (CC#36) was a gift from Connie Cepko (Addgene plasmid # 15163 ; http://n2t.net/addgene:15163 ; RRID:Addgene_15163)
For your References section:Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo. Fekete DM, Cepko CL. Mol Cell Biol. 1993 Apr . 13(4):2604-13. PubMed 8455633