Plasmid 13918: pSLAX13 (CT#232)

• Gene/insert name: None
• Vector backbone: Modified pBluescript
  (Search Vector Database)
• Vector type: Bacterial Expression
• Backbone size w/o insert: 3000
• 5' sequencing primer: T3
• 3' sequencing primer: T7
• Bacterial resistance: Ampicillin
• Growth strain: DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: Cliff Tabin
• Terms and Licenses: MTA

Comments: 

pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.

pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.

For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html

Article: Targeted gene misexpression in chick limb buds using avian replication-competent retroviruses. Logan et al (Methods. 1998 Apr . 14(4):407-20. PubMed)