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hsp70l: hAPOB48-mCherry.
(Plasmid #140639)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140639 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3156
  • Total vector size (bp) 13397
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    hsp70l
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1566
  • Entrez Gene
    hsp70l (a.k.a. HSP70, hsp70-4)
  • Promoter hsp70l

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    APOB
  • Species
    H. sapiens (human)
  • Mutation
    APOB48: amino acid 1-2180
  • Entrez Gene
    APOB (a.k.a. FCHL2, FLDB, LDLCQ4, apoB-100, apoB-48)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGG GAC AAG TTT GTA CAA AAA AGC AGG CTC AAT GGA CCC GCC GAG GCC C
  • 3′ sequencing primer GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC TTG TAT CAT ATA TGT CTG CAG TTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hsp70l: hAPOB48-mCherry. was a gift from Steven Farber (Addgene plasmid # 140639 ; http://n2t.net/addgene:140639 ; RRID:Addgene_140639)
  • For your References section:

    Model systems to study assembly, trafficking and secretion of apoB-lipoproteins using fluorescent fusion proteins. Walsh MT, Celestin OM, Thierer JH, Rajan S, Farber SA, Hussain MM. J Lipid Res. 2019 Dec 30. pii: jlr.RA119000259. doi: 10.1194/jlr.RA119000259. 10.1194/jlr.RA119000259 PubMed 31888978