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pET20b-delta133HtrA2
(Plasmid #14126)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14126 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET20b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3584
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    When transformed and plated on LB/agar/Amp plates, this plasmid will normally only produce colonies if the plates are incubated at room temp as opposed to 37oC. Following this, the bacteria when inoculated in liquid media will grow well at 37oC.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HtrA2
  • Alt name
    Omi
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    978
  • GenBank ID
    NM_013247
  • Entrez Gene
    HTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET20b-delta133HtrA2 was a gift from L. Miguel Martins (Addgene plasmid # 14126 ; http://n2t.net/addgene:14126 ; RRID:Addgene_14126)
  • For your References section:

    Binding specificity and regulation of the serine protease and PDZ domains of HtrA2/Omi. Martins LM, Turk BE, Cowling V, Borg A, Jarrell ET, Cantley LC, Downward J. J Biol Chem. 2003 Dec 5. 278(49):49417-27. 10.1074/jbc.M308659200 PubMed 14512424