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pAd-Track Flag HA PGC-1 alpha
(Plasmid #14426)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14426 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAdTrack
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Mammalian Expression, Adenoviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1 alpha
  • Alt name
    PPAR gamma coactivator 1
  • Alt name
    PGC1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Mutation
    Last four amino acids of PGC-1a removed to make the protein more stable.
  • GenBank ID
    NM_008904
  • Entrez Gene
    Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pAdTrack can be recombined with pAdEasy to make adenoviral-PGC1. See http://www.coloncancer.org/adeasy/protocol.htm

and He et al. http://www.pnas.org/cgi/content/full/95/5/2509

for protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-Track Flag HA PGC-1 alpha was a gift from Pere Puigserver (Addgene plasmid # 14426 ; http://n2t.net/addgene:14426 ; RRID:Addgene_14426)
  • For your References section:

    GCN5 acetyltransferase complex controls glucose metabolism through transcriptional repression of PGC-1alpha. Lerin C, Rodgers JT, Kalume DE, Kim SH, Pandey A, Puigserver P. Cell Metab. 2006 Jun . 3(6):429-38. 10.1016/j.cmet.2006.04.013 PubMed 16753578