- Backbone size w/o insert (bp) 9220
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesM. musculus (mouse)
Insert Size (bp)2300
Entrez GeneSirt1 (a.k.a. AA673258, SIR2L1, Sir2, Sir2a, Sir2alpha)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer No (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAd-Track Flag-SIRT1 was a gift from Pere Puigserver (Addgene plasmid # 8438)
For your References section:Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P. Nature 2005 Mar 3;434(7029):113-8. 10.1038/nature03354 PubMed 15744310
Map generated by Addgene from plasmid data.