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pAd-Track Flag-SIRT1
(Plasmid #8438)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAd-Track-CMV
  • Backbone size w/o insert (bp) 9220
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    SIRT1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Entrez Gene
    Sirt1 (a.k.a. AA673258, SIR2L1, Sir2, Sir2a, Sir2alpha)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer No
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-Track Flag-SIRT1 was a gift from Pere Puigserver (Addgene plasmid # 8438)
  • For your References section:

    Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P. Nature 2005 Mar 3;434(7029):113-8. 10.1038/nature03354 PubMed 15744310