Addgene - pAd-Track SIRT1 Plasmid Data

Plasmid 8440: pAd-Track SIRT1

Gene/insert name:
SIRT1
Insert size:
2200
Species:
M. musculus (mouse)
Entrez Gene:
Sirt1 (AA673258, SIR2L1, Sir2, Sir2a, Sir2alpha)
Vector backbone:
pAd-Track-CMV
(Search Vector Database)
Vector type:
Mammalian Expression, Adenoviral
Backbone size w/o insert (bp):
9220
Cloning site 5':
Kpn I
Site destroyed during cloning:
No
Cloning site 3':
Xho I
Site destroyed during cloning:
No
5' sequencing primer:
No List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Pere Puigserver
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Rodgers et al (Nature 2005 Mar 3;434(7029):113-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8440" in your Materials and Methods section.