Addgene - pIS47 C20orf139 3'UTR mut Plasmid Data

Plasmid 14490: pIS47 C20orf139 3'UTR mut

Gene/insert name:
C20orf139 3'UTR mutant
Alt name:
C20orf139
Insert size:
520
Species:
H. sapiens (human)
Entrez Gene:
SRXN1 (Npn3, SRX1, YKL086W, FLJ43353, C20orf139, dJ850E9.2)
Fusion protein or tag:
luciferase
Terminal:
N terminal on backbone
Mutation:
CATTCC’s were changed to AAGTAC's
Vector backbone:
pIS1
(Search Vector Database)
Backbone manufacturer:
Bartel Lab
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4085
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
EBV rev primer (GTGGTTTGTCCAAACTCATC)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

C20orf139 3'UTR mutant in renilla luciferase reporter plasmid.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Lim et al (Nature. 2005 Feb 17. 433(7027):769-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14490" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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