Addgene - pAG17 IQGAP1 3'UTR Plasmid Data

Plasmid 14503: pAG17 IQGAP1 3'UTR

Gene/insert name:
IQGAP1 3'UTR
Alt name:
IQGAP1
Insert size:
820
Species:
H. sapiens (human)
Fusion protein or tag:
luciferase
Terminal:
N terminal on backbone
Vector backbone:
pIS2
(Search Vector Database)
Backbone manufacturer:
pIS2 derived from pRL-SV40 (Promega)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
3700
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
EBV rev primer (GTGGTTTGTCCAAACTCATC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

IQGAP1 3'UTR in renilla luciferase reporter plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Lim et al (Nature. 2005 Feb 17. 433(7027):769-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14503" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

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