pAG30 IQGAP1 3'UTR mut
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14504||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerpIS2 derived from pRL-SV40 (Promega)
- Backbone size w/o insert (bp) 3700
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIQGAP1 3'UTR mutant
SpeciesH. sapiens (human)
Insert Size (bp)820
MutationGTGCCTT was changed to GTGCGCC and GTGCCT was changed to GTCGGT
Entrez GeneIQGAP1 (a.k.a. HUMORFA01, SAR1, p195)
/ Fusion Protein
- luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer EBV rev primer (GTGGTTTGTCCAAACTCATC) (Common Sequencing Primers)
IQGAP1 3'UTR mutant in renilla luciferase reporter plasmid. There are several sequence discrepancies between Addgene's quality control sequence and depositor's sequence. Please refer to the Addgene sequence for the correct mutations.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAG30 IQGAP1 3'UTR mut was a gift from David Bartel (Addgene plasmid # 14504 ; http://n2t.net/addgene:14504 ; RRID:Addgene_14504)
For your References section:Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, Castle J, Bartel DP, Linsley PS, Johnson JM. Nature. 2005 Feb 17. 433(7027):769-73. 10.1038/nature03315 PubMed 15685193