Addgene - LZRS-HOTAIR Plasmid Data

Plasmid 26110: LZRS-HOTAIR

Gene/insert name:
HOTAIR
Insert size:
2146
Species:
H. sapiens (human)
Entrez Gene:
HOTAIR (HOXAS, FLJ41747, HOXC11-AS1, NCRNA00072)
Vector backbone:
LZRS
(Search Vector Database)
Vector type:
Retroviral
Backbone size w/o insert (bp):
11100
Cloning method:
Gateway Cloning
Cloning site 5':
attR
Site destroyed during cloning:
Yes
Cloning site 3':
attR
Site destroyed during cloning:
Yes
5' sequencing primer:
TGGATACACGCCGCCCACGTG List of Sequencing Primers
3' sequencing primer:
ATCGTCGACCACTGTGCTGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
37
Growth instructions:
STBL2
High or low copy:
Low Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Howard Chang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Gupta et al (Nature. 2010 Apr 15. 464(7291):1071-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26110" in your Materials and Methods section.