Addgene - pLV.ATMi Plasmid Data

Plasmid 14542: pLV.ATMi

Gene/insert name:
ATM shRNA
Alt name:
ATM
Insert size:
60
Species:
H. sapiens (human)
Entrez Gene:
ATM (AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
Vector backbone:
pRDI292
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration. Ariumi et al (J Virol. 2005 Mar . 79(5):2973-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14542" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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