|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14581||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3176
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameATM shRNA
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GeneATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSUPER.ATMi was a gift from Didier Trono (Addgene plasmid # 14581 ; http://n2t.net/addgene:14581 ; RRID:Addgene_14581)
For your References section:DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration. Ariumi Y, Turelli P, Masutani M, Trono D. J Virol. 2005 Mar . 79(5):2973-8. 10.1128/JVI.79.5.2973-2978.2005 PubMed 15709017