Plasmid 14690: pRK5 bovine GRK5

• Gene/insert name: GRK5
• Alt name: G protein-coupled receptor kinase 5
• Alt name: GPRK5
• Insert size: 1900
• Species: B. taurus (bovine)
• Entrez Gene: GRK5 (GPRK5)
  
• Vector backbone: pRK5
  (Search Vector Database)
• Vector type: Mammalian Expression
• Backbone size w/o insert: 4700
• Cloning site 5': EcoRI
• Site destroyed during cloning: No
• Cloning site 3': XbaI
• Site destroyed during cloning: No
• 5' sequencing primer: Sp6
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: Robert Lefkowitz
• Terms and Licenses: MTA

Comments: 

The cDNA encoding bovine GRK5 was modified to remove all 3' and 5' untranslated regions. Using standard polymerase chain reaction techniques, a new 5' end containing EcoRI, BglII, and Kozak consensus sequences was added before the start codon, and a new 3' end containing an XbaI site was inserted immediately after the translational stop codon. The amplified fragment was inserted into the EcoRI/XbaI sites of pRK5.

Article: Phosphatidylinositol 4,5-bisphosphate (PIP2)-enhanced G protein-coupled receptor kinase (GRK) activity. Location, structure, and regulation of the PIP2 binding site distinguishes the GRK subfamilies. Pitcher et al (J Biol Chem. 1996 Oct 4. 271(40):24907-13. PubMed)