Addgene - pcDNA3.1 Hmga2 m2 Plasmid Data

Plasmid 14790: pcDNA3.1 Hmga2 m2

Gene/insert name:
Hmga2 m2
Alt name:
HMGA2
Insert size:
3700
Species:
M. musculus (mouse)
GenBank ID:
BC052158
Entrez Gene:
Hmga2 (9430083A20Rik, HMGI-C, Hmgic, pg, pygmy)
Mutation:
Hmga2 m2 mutant (see article and author's map for details)
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
David Bartel
Terms and Licenses:
MTA

Comments:

The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen).

Full sequence of wild-type plasmid available at Addgene plasmid #14789.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Disrupting the pairing between let-7 and Hmga2 enhances oncogenic transformation. Mayr et al (Science. 2007 Mar 16. 315(5818):1576-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14790" in your Materials and Methods section.