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pPERPluc1
(Plasmid #14810)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    PERP promoter
  • Alt name
    PERP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4000
  • Entrez Gene
    Perp (a.k.a. THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

The pPERPluc1 reporter was constructed by site-directed mutagenesis of the initial ATG of the PERP coding region (to create an NcoI site) using the Quick Change Site Directed mutagenesis kit (Stratagene), followed by joining of the 4-kb PERP promoter region to the initial ATG of luciferase in the pGL3Basic vector (Promega).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPERPluc1 was a gift from Tyler Jacks (Addgene plasmid # 14810)
  • For your References section:

    Multiple response elements and differential p53 binding control Perp expression during apoptosis. Reczek EE, Flores ER, Tsay AS, Attardi LD, Jacks T. Mol Cancer Res. 2003 Dec . 1(14):1048-57. PubMed 14707288