Addgene - pPERPluc1 Plasmid Data

Plasmid 14810: pPERPluc1

Gene/insert name:
PERP promoter
Alt name:
PERP
Insert size:
4000
Species:
M. musculus (mouse)
Entrez Gene:
Perp (THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik)
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Vector backbone:
pGL3-Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4800
5' sequencing primer:
RVprimer3 List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

The pPERPluc1 reporter was constructed by site-directed mutagenesis of the initial ATG of the PERP coding region (to create an NcoI site) using the Quick Change Site Directed mutagenesis kit (Stratagene), followed by joining of the 4-kb PERP promoter region to the initial ATG of luciferase in the pGL3Basic vector (Promega).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multiple response elements and differential p53 binding control Perp expression during apoptosis. Reczek et al (Mol Cancer Res. 2003 Dec . 1(14):1048-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14810" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

14811: pPERPlucFL

10838: pcDNA3 flag p53

11619: pLB

12089: pSico p53

12091: GFP-p53