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Plasmid 14810: pPERPluc1
  • PERP promoter

  • PERP

  • 4000

  • M. musculus (mouse)

  • Perp (THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik)

  • Luciferase

  • C terminal on backbone

  • pGL3-Basic
    (Search Vector Database)

  • Promega

  • Bacterial Expression

  • 4800

  • RVprimer3 List of Sequencing Primers

  • LucNrev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Tyler Jacks

  • MTA
    Luciferase Limited Use Label License

Comments: 

The pPERPluc1 reporter was constructed by site-directed mutagenesis of the initial ATG of the PERP coding region (to create an NcoI site) using the Quick Change Site Directed mutagenesis kit (Stratagene), followed by joining of the 4-kb PERP promoter region to the initial ATG of luciferase in the pGL3Basic vector (Promega).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multiple response elements and differential p53 binding control Perp expression during apoptosis. Reczek et al (Mol Cancer Res. 2003 Dec . 1(14):1048-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14810" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only