Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14810||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePERP promoter
SpeciesM. musculus (mouse)
Insert Size (bp)4000
Entrez GenePerp (a.k.a. 1110017A08Rik, KCP1, KRTCAP1, PIGPC1, THW)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
The pPERPluc1 reporter was constructed by site-directed mutagenesis of the initial ATG of the PERP coding region (to create an NcoI site) using the Quick Change Site Directed mutagenesis kit (Stratagene), followed by joining of the 4-kb PERP promoter region to the initial ATG of luciferase in the pGL3Basic vector (Promega).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPERPluc1 was a gift from Tyler Jacks (Addgene plasmid # 14810 ; http://n2t.net/addgene:14810 ; RRID:Addgene_14810)
For your References section:Multiple response elements and differential p53 binding control Perp expression during apoptosis. Reczek EE, Flores ER, Tsay AS, Attardi LD, Jacks T. Mol Cancer Res. 2003 Dec . 1(14):1048-57. PubMed 14707288