Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

pPERPlucFL
(Plasmid #14811)

Loading...
Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    PERP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8000
  • Entrez Gene
    Perp (a.k.a. THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

The pPERPlucFL vector was constructed by insertion of a splice acceptor upstream of the luciferase cassette in pGL3Basic and insertion of a BamHI Perp genomic fragment (from -3850 to +4150) into the polylinker region.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPERPlucFL was a gift from Tyler Jacks (Addgene plasmid # 14811)
  • For your References section:

    Multiple response elements and differential p53 binding control Perp expression during apoptosis. Reczek EE, Flores ER, Tsay AS, Attardi LD, Jacks T. Mol Cancer Res. 2003 Dec . 1(14):1048-57. PubMed 14707288