Addgene - pRRL_H1shmp53_PGK_eGFP_Teto Plasmid Data

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Plasmid 22039: pRRL_H1shmp53_PGK_eGFP_Teto

Gene/insert name:
shmp53
Insert size:
57
Vector backbone:
pRRL.SIN.cPPT.PGK.GFP
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert:
7600
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
HB101
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

shmp53 oligo sequence is: 5'-aaaaacactacaagtacatgtgtatttctcttgatacacatgtacttgtagtg

Please note that this plasmid is not hazardous to humans by itself, but virus made from the plasmid should be treated carefully. The shRNA it produces is against mouse p53 and the targeted sequence does not match with the human sequence. However since the degradation of human p53 would potentially have oncogenic consequences, viral stocks should be manipulated carefully.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22039" in your Materials and Methods section.