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pK18-hACE2
(Plasmid #149449)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 149449 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    pSEAP
  • Backbone manufacturer
    Tropix, Bedford, MA
  • Backbone size w/o insert (bp) 7114
  • Total vector size (bp) 9532
  • Modifications to backbone
    The vector includes 5' and 3' genomic regions of the human K18 gene
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Angiotensin-converting enzyme 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2418
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Promoter K18

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The vector includes 5' and 3' genomic regions of the human K18 gene, necessary for driving high-level epithelial-cell-specific expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK18-hACE2 was a gift from Paul McCray (Addgene plasmid # 149449 ; http://n2t.net/addgene:149449 ; RRID:Addgene_149449)
  • For your References section:

    Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus. McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S. J Virol. 2007 Jan;81(2):813-21. doi: 10.1128/JVI.02012-06. Epub 2006 Nov 1. 10.1128/JVI.02012-06 PubMed 17079315