PurposeIn vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||149707||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCR4 Blut TOPO
- Backbone size w/o insert (bp) 3947
- Total vector size (bp) 4653
Modifications to backboneAlphan globin 5'UTR is added at the downstream of T7 promoter Tandem human beta globin 3' UTR is added
Vector typeRNA transcription
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)387
Mutationdeleted amino acid 14-301
Entrez GeneTrp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (not destroyed)
- 3′ cloning site Asc (not destroyed)
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Revrse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid contains a ccdB marker in the backbone which appears to be nonfunctional/untranscribed under routine laboratory propagation in E. coli. If problems during routine propagation arise, the use of a ccdB tolerant strain is suggested.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:phBG-P53DD was a gift from Erika Sasaki (Addgene plasmid # 149707 ; http://n2t.net/addgene:149707 ; RRID:Addgene_149707)