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phBG-P53DD
(Plasmid #149707)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 149707 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCR4 Blut TOPO
  • Backbone size w/o insert (bp) 3947
  • Total vector size (bp) 4653
  • Modifications to backbone
    Alphan globin 5'UTR is added at the downstream of T7 promoter Tandem human beta globin 3' UTR is added
  • Vector type
    RNA transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trp53
  • Alt name
    P53
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    387
  • Mutation
    deleted amino acid 14-301
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Asc (not destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Revrse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs-p53DD (Addgene#22729)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a ccdB marker in the backbone which appears to be nonfunctional/untranscribed under routine laboratory propagation in E. coli. If problems during routine propagation arise, the use of a ccdB tolerant strain is suggested.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phBG-P53DD was a gift from Erika Sasaki (Addgene plasmid # 149707 ; http://n2t.net/addgene:149707 ; RRID:Addgene_149707)