Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #149707)


Item Catalog # Description Quantity Price (USD)
Plasmid 149707 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pCR4 Blut TOPO
  • Backbone size w/o insert (bp) 3947
  • Total vector size (bp) 4653
  • Modifications to backbone
    Alphan globin 5'UTR is added at the downstream of T7 promoter Tandem human beta globin 3' UTR is added
  • Vector type
    RNA transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    deleted amino acid 14-301
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Asc (not destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Revrse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs-p53DD (Addgene#22729)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a ccdB marker in the backbone which appears to be nonfunctional/untranscribed under routine laboratory propagation in E. coli. If problems during routine propagation arise, the use of a ccdB tolerant strain is suggested.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phBG-P53DD was a gift from Erika Sasaki (Addgene plasmid # 149707 ; ; RRID:Addgene_149707)