pEBG p130cas
(Plasmid #15001)

• Depositing Lab: Raymond Birge
• Publication: Zvara et al Oncogene. 2001 Feb 22. 20(8):951-61.

Backbone

• Vector backbone: pEBG
• Backbone manufacturer: Bruce Mayer Lab
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p130cas
• Alt name: p130 cas
• Species: R. norvegicus (rat)
• Entrez Gene: Bcar1 (a.k.a. Cas, Crkas, P130CAS)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: p130cas was originally from Mayer, and we made a GST fusion.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry