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Plasmid 15030: pSP64T (DM#111)
  • beta-globin 5' and 3' UTR

  • beta-globin

  • 260

  • X. laevis (frog)

  • pSP64
    (Search Vector Database)

  • Promega

  • Bacterial Expression

  • 3000

  • HindIII

  • No

  • XbaI

  • No

  • SP6 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • View map

  • Douglas Melton

  • UBMTA

Comments: 

Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Krieg et al (Nucleic Acids Res. 1984 Sep 25. 12(18):7057-70. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15030" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only