Addgene - M77 pIRESpuro-GLUE-hDsh3 Plasmid Data

Plasmid 15102: M77 pIRESpuro-GLUE-hDsh3

Gene/insert name:
Dsh3
Alt name:
Dishevelled 3
Insert size:
2200
Species:
H. sapiens (human)
Entrez Gene:
DVL3 ()
Fusion protein or tag:
streptag
Terminal:
N terminal on backbone
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Fusion protein or tag:
CBP tag
Terminal:
N terminal on backbone
Vector backbone:
pIRESpuro-GLUE
(Search Vector Database)
Backbone manufacturer:
Moon Lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Map:
View map
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation. Angers et al (Nat Cell Biol. 2006 Apr . 8(4):348-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15102" in your Materials and Methods section.