Chx10 3kb AP (CC#318)
(Plasmid #15205)

• Depositing Lab: Connie Cepko
• Publication: Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chx10 3kb AP reporter
• Alt name: Chx10
• Alt name: alkaline phosphatase
• Species: M. musculus (mouse)
• Insert Size (bp): 3000
• Entrez Gene: Vsx2 (a.k.a. Chx10, Hox-10, Hox10, or)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: SacII (destroyed during cloning)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 3Kb-AP reporter was constructed using a
genomic BamHI–SacII fragment isolated from a Chx10
BAC (Rowan and Cepko, 2004) and subcloned into pBluescript
(Stratagene). An SV40 intervening sequence (IVS)
was isolated from pNASSbeta (Clontech) digested with EcoRI
and blunt cloned into the 3' SacII restriction site. The full length alkaline phosphatase (AP) reporter construct was
isolated from the SV40all plasmid (gift of S. Dymecki).