|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20987||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1100
MutationN51A (makes DNA binding mutant protein)
Entrez GeneHOXB4 (a.k.a. HOX-2.6, HOX2, HOX2F)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
A possible N->A mutation was deemed acceptable due to its location on the homeodomain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HOXB4-NA-MSCV was a gift from Corey Largman (Addgene plasmid # 20987 ; http://n2t.net/addgene:20987 ; RRID:Addgene_20987)
For your References section:Cellular proliferation and transformation induced by HOXB4 and HOXB3 proteins involves cooperation with PBX1. Krosl J, Baban S, Krosl G, Rozenfeld S, Largman C, Sauvageau G. Oncogene. 1998 Jul 2. 16(26):3403-12. 10.1038/sj.onc.1201883 PubMed 9692548
Map uploaded by the depositor.