LB124 (Bcl-2 from -1287 to -3934)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15240||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5553
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Gene/Insert nameB-cell lymphoma/leukemia-2
SpeciesH. sapiens (human)
Insert Size (bp)2647
MutationBcl-2 fragment from -1287 to -3934 with P1
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Pst 1 (not destroyed)
- 3′ cloning site Pst 1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LB124 (Bcl-2 from -1287 to -3934) was a gift from Linda Boxer (Addgene plasmid # 15240 ; http://n2t.net/addgene:15240 ; RRID:Addgene_15240)
For your References section:Induction of bcl-2 expression by phosphorylated CREB proteins during B-cell activation and rescue from apoptosis. Wilson BE, Mochon E, Boxer LM. Mol Cell Biol. 1996 Oct . 16(10):5546-56. PubMed 8816467
Map uploaded by the depositor.