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LB124 (Bcl-2 from -1287 to -3934)
(Plasmid #15240)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15240 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBSKS+
  • Backbone size w/o insert (bp) 5553
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    B-cell lymphoma/leukemia-2
  • Alt name
    Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2647
  • Mutation
    Bcl-2 fragment from -1287 to -3934 with P1
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pst 1 (not destroyed)
  • 3′ cloning site Pst 1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LB124 (Bcl-2 from -1287 to -3934) was a gift from Linda Boxer (Addgene plasmid # 15240 ; http://n2t.net/addgene:15240 ; RRID:Addgene_15240)
  • For your References section:

    Induction of bcl-2 expression by phosphorylated CREB proteins during B-cell activation and rescue from apoptosis. Wilson BE, Mochon E, Boxer LM. Mol Cell Biol. 1996 Oct . 16(10):5546-56. PubMed 8816467