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LB322 (Bcl-2 from ATG to -3934)
(Plasmid #15381)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15381 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS(+)
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    B-cell lymphoma/leukemia-2
  • Alt name
    Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3926
  • Mutation
    Bcl-2 fragment from ATG to -3934 with both P1 and P2
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LB322 (Bcl-2 from ATG to -3934) was a gift from Linda Boxer (Addgene plasmid # 15381 ; http://n2t.net/addgene:15381 ; RRID:Addgene_15381)
  • For your References section:

    A-Myb up-regulates Bcl-2 through a Cdx binding site in t(14;18) lymphoma cells. Heckman CA, Mehew JW, Ying GG, Introna M, Golay J, Boxer LM. J Biol Chem. 2000 Mar 3. 275(9):6499-508. 10.1074/jbc.275.9.6499 PubMed 10692454