LB322 (Bcl-2 from ATG to -3934)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15381||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS(+)
- Backbone size w/o insert (bp) 6000
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Gene/Insert nameB-cell lymphoma/leukemia-2
SpeciesH. sapiens (human)
Insert Size (bp)3926
MutationBcl-2 fragment from ATG to -3934 with both P1 and P2
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LB322 (Bcl-2 from ATG to -3934) was a gift from Linda Boxer (Addgene plasmid # 15381 ; http://n2t.net/addgene:15381 ; RRID:Addgene_15381)
For your References section:A-Myb up-regulates Bcl-2 through a Cdx binding site in t(14;18) lymphoma cells. Heckman CA, Mehew JW, Ying GG, Introna M, Golay J, Boxer LM. J Biol Chem. 2000 Mar 3. 275(9):6499-508. 10.1074/jbc.275.9.6499 PubMed 10692454
Map uploaded by the depositor.