LB556 (Bcl-2 from ATG to -170)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15383||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBS II KS+
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Gene/Insert nameB-cell lymphoma/leukemia-2
SpeciesH. sapiens (human)
Insert Size (bp)162
MutationBcl-2 fragment from ATG to -170 with P2
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Sty1 (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LB556 (Bcl-2 from ATG to -170) was a gift from Linda Boxer (Addgene plasmid # 15383 ; http://n2t.net/addgene:15383 ; RRID:Addgene_15383)
Map uploaded by the depositor.