Addgene - LB556 (Bcl-2 from ATG to -170) Plasmid Data

Plasmid 15383: LB556 (Bcl-2 from ATG to -170)

Gene/insert name:
B-cell lymphoma/leukemia-2
Alt name:
Bcl-2
Insert size:
162
Species:
H. sapiens (human)
Entrez Gene:
BCL2 (Bcl-2, PPP1R50)
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Mutation:
Bcl-2 fragment from ATG to -170 with P2
Vector backbone:
pBS II KS+
(Search Vector Database)
Vector type:
Bacterial Expression, Luciferase
Backbone size w/o insert (bp):
3000
Cloning site 5':
Sty1
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Linda Boxer
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15383" in your Materials and Methods section.