Addgene - LB360 (Bcl-2 from -1287 to -1337) Plasmid Data

Plasmid 15242: LB360 (Bcl-2 from -1287 to -1337)

Gene/insert name:
B-cell lymphoma/leukemia-2
Alt name:
Bcl-2
Insert size:
50
Species:
H. sapiens (human)
Entrez Gene:
BCL2 (Bcl-2, PPP1R50)
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Mutation:
Bcl-2 fragment from -1287 to -1337 with P1
Vector backbone:
pBSKS+
(Search Vector Database)
Vector type:
Bacterial Expression, Luciferase
Backbone size w/o insert (bp):
5620
Cloning site 5':
ApaI
Site destroyed during cloning:
No
Cloning site 3':
Pst1
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Linda Boxer
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of bcl-2 expression by phosphorylated CREB proteins during B-cell activation and rescue from apoptosis. Wilson et al (Mol Cell Biol. 1996 Oct . 16(10):5546-56. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15242" in your Materials and Methods section.