Addgene - LB1554 (Bcl-2 promoter with MHS1234, Cdx mut) Plasmid Data

Plasmid 15384: LB1554 (Bcl-2 promoter with MHS1234, Cdx mut)

Gene/insert name:
B-cell lymphoma/leukemia-2
Alt name:
Bcl-2
Insert size:
10501
Species:
H. sapiens (human)
Entrez Gene:
BCL2 (Bcl-2, PPP1R50)
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Mutation:
Bcl-2 full promoter with IgH full enhancer MHS1234 with Cdx mutation at MHS12 (ATTT -> CGCC)
Vector backbone:
pREP4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Bacterial Expression, Luciferase
Backbone size w/o insert (bp):
10183
Cloning site 5':
See map
Site destroyed during cloning:
No
Cloning site 3':
See map
Site destroyed during cloning:
No
5' sequencing primer:
pREP_fwd List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Hygromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Linda Boxer
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The immunoglobulin heavy-chain gene 3' enhancers deregulate bcl-2 promoter usage in t(14;18) lymphoma cells. Duan et al (Oncogene. 2007 Apr 19. 26(18):2635-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15384" in your Materials and Methods section.