LB1461 (Bcl-2 promoter with MHS1234, NFkB mut)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15385||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10183
Vector typeMammalian Expression, Bacterial Expression, Luciferase
Growth in Bacteria
Gene/Insert nameB-cell lymphoma/leukemia-2
SpeciesH. sapiens (human)
Insert Size (bp)10502
MutationBcl-2 full promoter with IgH full enhancer MHS1234 with NF-kB mutation at MHS12
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer pREP_fwd (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LB1461 (Bcl-2 promoter with MHS1234, NFkB mut) was a gift from Linda Boxer (Addgene plasmid # 15385 ; http://n2t.net/addgene:15385 ; RRID:Addgene_15385)
For your References section:The immunoglobulin heavy-chain gene 3' enhancers deregulate bcl-2 promoter usage in t(14;18) lymphoma cells. Duan H, Heckman CA, Boxer LM. Oncogene. 2007 Apr 19. 26(18):2635-41. 10.1038/sj.onc.1210061 PubMed 17043638
Map uploaded by the depositor.