PB-CAG-CasRX-P2A-EGFP-BGH PolyA
(Plasmid #154004)

• Purpose: Express CasRx
• Depositing Lab: Hui Yang
• Publication: He et al Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2.

Backbone

• Vector backbone: PB
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CasRx
• Alt name: NLS-RfxCas13d-NLS
• Species: Synthetic
• Insert Size (bp): 5753
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • 5 SP123.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

INSERT: CasRx & EGFP driven by CAG promoter.