human TF cDNA
(Plasmid #15455)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pUC8
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 2665
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Tissue factor
  • Alt name
    TF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    641
  • Entrez Gene
    F3 (a.k.a. CD142, TF, TFA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13-F20
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Mackman lab pCTF 314.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human TF cDNA was a gift from Nigel Mackman (Addgene plasmid # 15455)
  • For your References section:

    Regulation of the tissue factor gene. Mackman N. FASEB J. 1995 Jul . 9(10):883-9. PubMed 7615158