Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15475||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namenorepinephrine transporter
SpeciesH. sapiens (human)
Insert Size (bp)1900
Entrez GeneSLC6A2 (a.k.a. NAT1, NET, NET1, SLC6A5)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hNET pcDNA3 was a gift from Randy Blakely (Addgene plasmid # 15475 ; http://n2t.net/addgene:15475 ; RRID:Addgene_15475)
For your References section:Sodium-dependent norepinephrine-induced currents in norepinephrine-transporter-transfected HEK-293 cells blocked by cocaine and antidepressants. Galli A, DeFelice LJ, Duke BJ, Moore KR, Blakely RD. J Exp Biol. 1995 Oct . 198(Pt 10):2197-212. PubMed 7500004