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hSERT pcDNA3
(Plasmid #15483)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15483 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSERT
  • Alt name
    serotonin transporter
  • Alt name
    5HT transporter
  • Alt name
    solute carrier family 6 member 4 (SLC6A4)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Entrez Gene
    SLC6A4 (a.k.a. 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSERT pcDNA3 was a gift from Randy Blakely (Addgene plasmid # 15483 ; http://n2t.net/addgene:15483 ; RRID:Addgene_15483)
  • For your References section:

    Antidepressant- and cocaine-sensitive human serotonin transporter: molecular cloning, expression, and chromosomal localization. Ramamoorthy S, Bauman AL, Moore KR, Han H, Yang-Feng T, Chang AS, Ganapathy V, Blakely RD. Proc Natl Acad Sci U S A. 1993 Mar 15. 90(6):2542-6. 10.1073/pnas.90.6.2542 PubMed 7681602