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hChT pcDNA3
(Plasmid #15766)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15766 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hemicholinium-3-sensitive choline transporter
  • Alt name
    ChT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Entrez Gene
    SLC5A7 (a.k.a. CHT, CHT1, CMS20, HMN7A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hChT pcDNA3 was a gift from Randy Blakely (Addgene plasmid # 15766 ; http://n2t.net/addgene:15766 ; RRID:Addgene_15766)
  • For your References section:

    Molecular cloning of a human, hemicholinium-3-sensitive choline transporter. Apparsundaram S, Ferguson SM, George AL, Blakely RD. Biochem Biophys Res Commun. 2000 Oct 5. 276(3):862-7. 10.1006/bbrc.2000.3561 PubMed 11027560