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pWPI-IRES-Puro-Ak-ACE2
(Plasmid #154985)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154985 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Lentiviral Prep 154985-LV Virus (1mL at titer ≥ 1x10⁶ TU/mL) and Plasmid.
$130

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2418
  • Entrez Gene
    ACE2 (a.k.a. ACEH)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Information for Lentiviral Prep (Catalog # 154985-LV) ( Back to top )

Purpose

Ready-to-use Lentiviral Prep particles produced from pWPI-IRES-Puro-Ak-ACE2 (#154985). In addition to the viral particles, you will also receive purified pWPI-IRES-Puro-Ak-ACE2 plasmid DNA.

Lentiviral particles carrying human ACE2.

Delivery

  • Volume 1mL
  • Titer ≥ 1x10⁶ TU/mL
  • Pricing $100 USD for preparation of 1mL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids psPAX2 (plasmid #12260)
  • Envelope pMD2.G (plasmid #12259)
  • Buffer OptiPro

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Resource Information

Viral Quality Control

Titering Method:
  • ddPCR assay: 293T cells were transduced with serial dilutions of 154985-LV, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.
Notes:
  • PCR confirmation of insert: PCRs were carried out on the viral preparation with primers targeting the backbone and ACE2 sequences. The PCR products were visualized on an agarose gel for size confirmation.

Visit our viral production page for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPI-IRES-Puro-Ak-ACE2 was a gift from Sonja Best (Addgene plasmid # 154985 ; http://n2t.net/addgene:154985 ; RRID:Addgene_154985)

    For viral preps, please replace (Addgene plasmid # 154985) in the above sentence with: (Addgene viral prep # 154985-LV)