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Plasmid 36085: pLV-EBFP2-nuc
  • pEBFP2-Nuc

  • EBFP2 blue fluorescent protein with NLS

  • 720

  • Synthetic; evolved fluorescent protein

  • EF517318

  • NLS

  • C terminal on backbone

  • Replace EYFP of the vector pEYFP-Nuc from Clontech with EBFP2.

  • pLenti-MP2
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 6782

  • none

  • Unknown

  • BamHI

  • No

  • CMV fwd List of Sequencing Primers

  • WPRE-R

  • Ampicillin

  • Stbl3

  • 37

  • optimal growth in low salt LB (5gm NaCl)

  • Unknown

  • The EBFP2-nuc was obtained from Addgene and was originally from Robert Campbell's group (Ai HW et al. Biochemistry 2007).

  • View sequences (2)
  • Pantelis Tsoulfas

  • UBMTA

Comments: 

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36085" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only