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Plasmid 36085: pLV-EBFP2-nuc
  • pEBFP2-Nuc

  • EBFP2 blue fluorescent protein with NLS

  • 720

  • Synthetic; evolved fluorescent protein

  • EF517318

  • NLS

  • C terminal on backbone

  • Replace EYFP of the vector pEYFP-Nuc from Clontech with EBFP2.

  • pLenti-MP2
    (Search Vector Database)

  • Lentiviral

  • 6782

  • none

  • Unknown

  • BamHI

  • No

  • CMV fwd List of Sequencing Primers

  • WPRE-R

  • Ampicillin

  • Stbl3

  • 37

  • optimal growth in low salt LB (5gm NaCl)

  • Unknown

  • The EBFP2-nuc was obtained from Addgene and was originally from Robert Campbell's group (Ai HW et al. Biochemistry 2007).

  • View sequences (2)
  • Pantelis Tsoulfas

  • UBMTA

Comments: 

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36085" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only