Addgene - pLV-EBFP2-nuc Plasmid Data

Plasmid 36085: pLV-EBFP2-nuc

Gene/insert name:
pEBFP2-Nuc
Alt name:
EBFP2 blue fluorescent protein with NLS
Insert size:
720
Species:
Synthetic
Species:
evolved fluorescent protein
GenBank ID:
EF517318
Fusion protein or tag:
NLS
Terminal:
C terminal on backbone
Mutation:
Replace EYFP of the vector pEYFP-Nuc from Clontech with EBFP2.
Vector backbone:
pLenti-MP2
(Search Vector Database)
Vector type:
Lentiviral
Backbone size (bp):
6782
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV fwd List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
optimal growth in low salt LB (5gm NaCl)
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
The EBFP2-nuc was obtained from Addgene and was originally from Robert Campbell's group (Ai HW et al. Biochemistry 2007).
Sequence: View sequences (2)
Principal Investigator:
Pantelis Tsoulfas
Terms and Licenses:
MTA

Comments:

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36085" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

31856: pShuttle-CMV-iRFP