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pLV-EBFP2-nuc
(Plasmid #36085)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLenti-MP2
  • Backbone size (bp) 6782
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    optimal growth in low salt LB (5gm NaCl)
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    pEBFP2-Nuc
  • Alt name
    EBFP2 blue fluorescent protein with NLS
  • Species
    Synthetic; evolved fluorescent protein
  • Insert Size (bp)
    720
  • Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontech with EBFP2.
  • GenBank ID
    EF517318
  • Tag / Fusion Protein
    • NLS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The EBFP2-nuc was obtained from Addgene and was originally from Robert Campbell's group (Ai HW et al. Biochemistry 2007).
  • Terms and Licenses

Depositor Comments

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EBFP2-nuc was a gift from Pantelis Tsoulfas (Addgene plasmid # 36085)