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pCS2-QFGal4-SV40pA
(Plasmid #155126)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 155126 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4053
  • Total vector size (bp) 5055
  • Vector type
    Mammalian Expression ; Expression in zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    QFGal4
  • Species
    S. cerevisiae (budding yeast); Neurospora crassa
  • Insert Size (bp)
    1002
  • GenBank ID
    855828 3875756
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer ATT TAG GTG ACA CTA TAG
  • 3′ sequencing primer GGA TAA CAA TTT CAC ACA GG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Can be linearized with NotI for in vitro synthesis of QFGal4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-QFGal4-SV40pA was a gift from Bret Pearson (Addgene plasmid # 155126 ; http://n2t.net/addgene:155126 ; RRID:Addgene_155126)
  • For your References section:

    An optimized QF-binary expression system for use in zebrafish. Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ. Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 10.1016/j.ydbio.2020.07.007. 10.1016/j.ydbio.2020.07.007 PubMed 32697972